Myosin Heavy Chain Types and Expression Levels of Myostatin and Myogenin Genes in Muscle of Two Rabbit Breeds

نویسندگان

  • Lei
  • Ren
  • Zhen
  • Zhang
  • Guo
  • Zheng
چکیده

The aim of the present study was to compare fiber type compositions in terms of Myosin Heavy Chain (MyHC) and expression levels of myostatin (MSTN) and myogenin (MyoG) in longissimus dorsi of two rabbit breeds with different body sizes. Longissimus dorsi samples of Qixin rabbits (n=12) and German New Zealand of ZIKA rabbits (n=12) at 84d after birth (marketing age) were collected. Quantitative real-time PCR analysis revealed four types of fiber in longissimus dorsi, and MyHC-1 and MyHC-2D were the major types. No significant difference was observed in the proportions of MyHC isoforms in longissimus dorsi between the two breeds. In addition, the two rabbit breeds at 84d had showed non-significant mRNA differences in levels of MSTN and MyoG in longissimus dorsi. These results suggest that MyHC types, MSTN and MyoG expression may not directly associated with the different body sizes of the two rabbit breeds studied.

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تاریخ انتشار 2012